http://dx.doi.org/10.1016/j.talanta.2014.08.022">
 

Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Peptidomics study of anthocyanin-rich juice of elderberry

Language

English

Abstract

© 2014 Elsevier B.V. All rights reserved. Biologically active peptides play a role in plant signaling and defense. Elderberry juice is known to contain a variety of anthocyanin compounds, a sub-set of polyphenols, which are responsible for the deep purple color of the juice. In this paper, we describe a method utilizing solid phase extraction (SPE) to remove anthocyanins from peptides. Liquid chromatography coupled with tandem mass spectrometry was used to separate and identify the peptides. The results showed that the use of SPE was an effective method to separate peptides from anthocyanins and other background compounds including high polyphenol content in the juice samples. More than 1000 peptides present in elderberry juice were successfully identified.

Keywords

Anthocyanin sambucus, Elderberry, Orbitrap-MS/MS, Peptidomics, Solid phase extraction

Publication Date

2015

Source Publication Title

Talanta

Volume

131

Start Page

640

End Page

644

Publisher

Elsevier

ISSN (print)

00399140

ISSN (electronic)

18733573

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