http://dx.doi.org/10.1002/chem.200902919">
 

Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

A triphenylphosphonium-functionalised cyclometalated Platinum(II) complex as a nucleolus-specific two-photon molecular dye

Language

English

Abstract

An organometallic cyclometalated platinum(II) complex, [Pt(L 3)Cl] [PF6], has been synthesised from a specially designed cyclometalating ligand, HL3 (triphenyl{5-[3-(6- phenylpyridin-2-yl)-l//-pyrazol-1-yl]pentyl}phosphonium chloride), that contains a pendant carbon chain carrying a terminal cationic triphenylphosphonium moiety. Aside from its room temperature single-photon luminescent properties in solution, [Pt(L3)Cl] + can also produce two-photon-induced luminescence at room temperature upon excitation at 700 nm from a mode- locked Ti: sapphire laser. Its twophoton absorption cross-section in DMF at room temperature was measured to be 28.0 x 10-50cm4 s photon-1. [Pt(L3)Cl]+ is able to selectively stain the cell nucleolus. This has been demonstrated by two-photon confocal imaging of live and methanol-fixed HeLa (human cervical carcinoma) and 3T3 (mouse skin fibroblasts) cells. This organelle specificity is likely to be related to its special affinity for proteins within cell nucleoli. As a result of such protein affinity, [Pt(L3)Cl]+ is an efficient RNA transcription inhibitor and shows rather profound cytotoxicity. On the other hand, the organelle-specific labelling and two-photon-induced luminescent properties of [Pt(L3)Cl]+ renders it a useful nuclear dye for the 3-dimensional reconstruction of optical sections of thick tissues, for example, mouse ileum tissues, by multiphoton confocal microscopy. © 2010 Wiley-VCH. Verlag GmbH & Co. KGaA.

Keywords

Imaging agents, Optical tissue sectioning, Organelle-specific labeling, Platinum, Two-photon imaging

Publication Date

2010

Source Publication Title

Chemistry - A European Journal

Volume

16

Issue

13

Start Page

3942

End Page

3950

Publisher

Wiley

ISSN (print)

09476539

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