Department of Chemistry
A G-triplex luminescent switch-on probe for the detection of mung bean nuclease activity
© The Royal Society of Chemistry 2015.A cyclometallated Ir(III) complex was synthesised and evaluated for the ability to act as a luminescent G-triplex probe. The iridium(III) complex 1 [Ir(phq)2(2,9-dmphen)]PF6 (where phq = 2-phenylquinoline; 2,9-dmphen = 2,9-dimethyl-1,10-phenanthroline) exhibited high luminescence for G-triplex DNA over dsDNA and ssDNA, and was employed to construct a label-free G-triplex-based assay for mung bean nuclease activity in aqueous solution. This method was highly sensitive and selective for MB nuclease activity over other DNA-modifying enzymes.
Source Publication Title
Journal of Materials Chemistry B
Royal Society of Chemistry
Ma, Dik-Lung, Lihua Lu, Sheng Lin, Bingyong He, and Chung-Hang Leung. "A G-triplex luminescent switch-on probe for the detection of mung bean nuclease activity." Journal of Materials Chemistry B 3.3 (2015): 348-352.