Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Language

English

Abstract

Gastric cancer remains a major health problem worldwide. Natural products, with stronger antitumor activity and fewer side effects, are potential candidates for pharmaceutical development as anticancer agents. In this study, quercetin and curcumin were chosen for testing and were applied separately and in combination to human gastric cancer MGC-803 cells. The MTT assay was used to evaluate cell growth inhibition. Annexin V-FITC/PI was carried out to measure apoptosis rate. Flow cytometry was performed to analyze mitochondrial membrane potential levels. Western blots were applied to detect expression of cytochrome c, total and phosphorylated ERK and AKT. Combined treatment with curcumin and quercetin resulted in significant inhibition of cell proliferation, accompanied by loss of mitochondrial membrane potential (Ä∅m), release of cytochrome c and decreased phosphorylation of AKT and ERK. These results indicate that the combination of curcumin and quercetin induces apoptosis through the mitochondrial pathway. Notably, effect of combined treatment with curcumin and quercetin on gastric cancer MGC-803 cells is stronger than that of individual treatment, indicating that curcumin and quercetin combinations have potential as anti-gastric cancer drugs for further development.

Keywords

AKT, Apoptosis, Combined effect, Curcumin, ERK, Quercetin

Publication Date

6-2015

Source Publication Title

Molecules

Volume

20

Issue

6

Start Page

11524

End Page

11534

Publisher

MDPI

Peer Reviewed

1

Copyright

© 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license.

Funder

The work was supported by the Faculty Research Grant of Hong Kong Baptist University (FRG2/13-14/044), National Natural Science Foundation of China (No. 81473320), the Fund from Guangdong Science and Technology Department & Guangdong Academy of Traditional Chinese Medicine (2012A032500021) and the Fund of Guangzhou Science and Technology Program (2013J4100033).

DOI

10.3390/molecules200611524

Link to Publisher's Edition

http://dx.doi.org/10.3390/molecules200611524

ISSN (print)

14203049

ISSN (electronic)

14203049

Share

COinS