Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Language

English

Abstract

Background: Dioscorea is a genus of flowering plants, and some Dioscorea species are known and used as a source for the steroidal sapogenin diosgenin. To screen potential resource from Dioscorea species and related medicinal plants for diosgenin extraction, a rapid method to compare the contents of diosgenin in various plants is crucial. Results: An ultra-performance liquid chromatography (UPLC) coupled with diode array detection (DAD) and electrospray ionization mass spectrometry (ESI-MS) method was developed for identification and determination of diosgenin in various plants. A comprehensive validation of the developed method was conducted. Twenty-four batches of plant samples from four Dioscorea species, one Smilax species and two Heterosmilax species were analyzed by using the developed method.The present method presented good sensitivity, precision and accuracy. Diosgenin was found in three Dioscorea species and one Heterosmilax species, namely D. zingiberensis, D. septemloba, D. collettii and H. yunnanensis. Conclusion: The method is suitable for the screening of diosgenin resources from plants. D. zingiberensis is an important resource for diosgenin harvesting.

Keywords

Dioscorea, Diosgenin, Medicinal plants, Quality evaluation, UPLC-DAD-MS

Publication Date

8-2014

Source Publication Title

BMC Biochemistry

Volume

15

Start Page

19

Publisher

BioMed Central

Peer Reviewed

1

Copyright

© Yi et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

Funder

This research was funded by the Faculty Research Grant of Hong Kong Baptist University (FRG2/13-14/031 and FRG1/12-13/035), the Health Department of Guangxi province (Zhong2010111 and Z2010175) and Guangxi Administration of Traditional Chinese Medicine (GZKZ1131) of China.

DOI

10.1186/1471-2091-15-19

Link to Publisher's Edition

http://dx.doi.org/10.1186/1471-2091-15-19

ISSN (print)

14712091

ISSN (electronic)

14712091

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