Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Language

English

Abstract

Background: Rhizoma Chuanxiong (RC) is the dried rhizome ofLigusticum chuanxiongHort., and various types of processed Rhizoma Chuanxiong (PRC) are widely used in China. However, quality assurance and quality control of these processed medicines remain challenging. This study aims to investigate the chemical compositions of various PRC preparations by a high-performance liquid chromatography (HPLC) coupled with diode array detection (DAD) method.Methods: A HPLC-DAD method with validation was developed for PRC samples. Seven batches of plant samples from two processing methods, stir-frying and steaming, were analyzed by the HPLC-DAD method. Common peaks in PRC chromatograms were chosen to calculate their relative retention time (RRT) and relative peak area (RPA), and similarity analyses of the chromatographic fingerprints were conducted by Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine software (Version 2004 A).Results: In the 24-h stability test, the relative standard deviation for the RRT and RPA was less than 0.07% and 2.57%, respectively. The precision was less than 0.08% for the RRT and 2.48% for the RPA. The repeatability for the RRT and RPA was less than 0.03% and 2.64%, respectively. The similarities between the seven PRC batches were range from 0.956 to 0.990. After stir-frying or steaming, the amount of ferulic acid in PRC was much higher than that in the raw material.Conclusions: The fingerprint analysis of PRC by different processing methods was feasible by HPLC-DAD.

Keywords

Fingerprint, HPLC-DAD, Ligusticum chuanxiong, Processing, Rhizoma chuanxiong

Publication Date

2-2015

Source Publication Title

Chinese Medicine

Volume

10

Start Page

2

Publisher

BioMed Central

Peer Reviewed

1

Copyright

© Fang et al.; licensee BioMed Central. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

DOI

10.1186/s13020-015-0031-3

Link to Publisher's Edition

http://dx.doi.org/10.1186/s13020-015-0031-3

ISSN (print)

17498546

ISSN (electronic)

17498546

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