Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Highly sensitive graphene–pt nanocomposites amperometric biosensor and its application in living cell H2O2 detection

Language

English

Abstract

© 2014 American Chemical Society.A sensitive hydrogen peroxide (H2O2) sensor was constructed based on graphene-Pt (RGO-Pt) nanocomposites and used to measure the release of H2O2 from living cells. The graphene and Pt nanoparticles (Pt NPs) were modified on glassy carbon electrode (GCE) by the physical adsorption and electrodeposition of K2PtCl6 solution, respectively. Through characterization by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS), it was observed that the electrodeposited Pt NPs were densely covered and well distributed on the entire graphene surface. Electrochemical study demonstrates that the RGO-Pt nanocomposites modified glassy carbon electrode exhibited a high peak current and low overpotential toward the reduction of H2O2. The relevant detection limit of H2O2 is ∼0.2 μM with a wide linear range from 0.5 μM to 3.475 mM, displaying a much higher sensitivity (459 ± 3 mA M-1 cm-2, n = 5) than that of Pt nanoparticles or graphene modified electrode. This novel biosensor can measure the H2O2 release from living cells because of its low detection limit, wide linear range, and higher sensitivity.

Publication Date

2014

Source Publication Title

Analytical Chemistry

Volume

86

Issue

19

Start Page

9459

End Page

9465

Publisher

American Chemical Society

DOI

10.1021/ac5009699

Link to Publisher's Edition

http://dx.doi.org/10.1021/ac5009699

ISSN (print)

00032700

ISSN (electronic)

15206882

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