Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Title

Identification of Chinese herbal medicines by fluorescence microscopy: Fluorescent characteristics of medicinal bark

Language

English

Abstract

© 2014 Royal Microscopical Society. Summary: Medicinal bark refers to structures outside the vascular cambium of stems, branches and roots of gymnospermous and dicotyledonous plants that are used as medicinal materials; bark is an important type of Chinese herbal medicine. However, identification of the species from which the bark comes can be very difficult, especially when the bark is dried and sliced. In our previous studies, we have found that fluorescence microscopy is a powerful tool for the identification of easily confused Chinese herbal medicines, powdered Chinese herbal medicines and decoction dregs. To establish the fluorescent characteristics by which medicinal barks can be identified, for ensuring their safe and effective use, a systematic microscopic investigation by normal light and fluorescence microscope was carried out on transverse section samples of 11 medicinal barks commonly used in China. Specifically, the fluorescent characteristics of mechanical tissues, including stone cells and fibres as well as secretory tissues, have been observed. The microscopic features of medicinal bark are here systematically and comparatively described and illustrated. Under the fluorescence microscope, various tissues emitted fluorescence of different colours, and we found that both the colours and the intensity can be used to distinguish and identify these barks.

Keywords

Fibres, Fluorescence microscopy, Medicinal bark, Normal light microscopy, Secretory tissues, Stone cells

Publication Date

2014

Source Publication Title

Journal of Microscopy

Volume

256

Issue

1

Start Page

6

End Page

22

Publisher

Wiley

DOI

10.1111/jmi.12153

Link to Publisher's Edition

http://dx.doi.org/10.1111/jmi.12153

ISSN (print)

00222720

ISSN (electronic)

13652818

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