Department of Biology
Illumina synthetic long read sequencing allows recovery of missing sequences even in the "Finished" C. elegans genome
Most next-generation sequencing platforms permit acquisition of high-throughput DNA sequences, but the relatively short read length limits their use in genome assembly or finishing. Illumina has recently released a technology called Synthetic Long-Read Sequencing that can produce reads of unusual length, i.e., predominately around 10Kb. However, a systematic assessment of their use in genome finishing and assembly is still lacking. We evaluate the promise and deficiency of the long reads in these aspects using isogenic C. elegans genome with no gap. First, the reads are highly accurate and capable of recovering most types of repetitive sequences. However, the presence of tandem repetitive sequences prevents pre-assembly of long reads in the relevant genomic region. Second, the reads are able to reliably detect missing but not extra sequences in the C. elegans genome. Third, the reads of smaller size are more capable of recovering repetitive sequences than those of bigger size. Fourth, at least 40Kbp missing genomic sequences are recovered in the C. elegans genome using the long reads. Finally, an N50 contig size of at least 86Kbp can be achieved with 24×reads but with substantial mis-assembly errors, highlighting a need for novel assembly algorithm for the long reads.
Source Publication Title
Nature Publishing Group
Li, Runsheng, Chia-Ling Hsieh, Amanda Young, Zhihong Zhang, Xiaoliang Ren, and Zhongying Zhao. "Illumina synthetic long read sequencing allows recovery of missing sequences even in the "Finished" C. elegans genome." Scientific Reports 5 (2015): 10814.