School of Chinese Medicine
In situ synthesis of self-assembled three-dimensional graphene–magnetic palladium nanohybrids with dual-enzyme activity through one-pot strategy and its application in glucose probe
© 2015 American Chemical Society. The self-assembled three-dimensional graphene nanohybrids with in situ-formed Fe3O4 and Pd nanoparticles on it (3DRGO-Fe3O4-Pd) are first synthesized by the one-pot solvothermal method, which have intrinsic peroxidase-like and oxidase-like activity. The catalytic mechanism is analyzed by the electron spin resonance (ESR), fluorescence, and electrochemical methods. The mimic enzyme catalytic activity of 3DRGO-Fe3O4-Pd is much higher than those of monometallic loaded nanohybrids and their physical mixture, probably caused by synergistic effect between Pd and Fe3O4 nanoparticles. The 3DRGO-Fe3O4-Pd nanohybrids was reproducible, stable, and reusable. After 10 cycles, the catalytic activity was still higher than 90%, and the morphology and structure were basically unchanged. Based on its high peroxidase-like activity, especially the enhanced affinity toward H2O2, a new colorimetric detection method for reduced glutathione (GSH) and glucose has been designed using H2O2 as an intermediary, which provides a simple, sensitive, and selective way to detect urine glucose of diabetes with a wide linear range and low detection limit.
colorimetry, dual-enzyme activity, glucose, one-pot, three-dimensional graphene nanohybrids
Source Publication Title
ACS Applied Materials and Interfaces
American Chemical Society
Zheng, Xuejing, Qian Zhu, Huiqing Song, Xinrui Zhao, Tao Yi, Hongli Chen, and Xingguo Chen. "In situ synthesis of self-assembled three-dimensional graphene–magnetic palladium nanohybrids with dual-enzyme activity through one-pot strategy and its application in glucose probe." ACS Applied Materials and Interfaces 7.6 (2015): 3480-3491.