Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Label-free luminescent detection of LMP1 gene deletion using an intermolecular G-quadruplex-based switch-on probe

Language

English

Abstract

© 2015 Elsevier B.V. We have synthesized a series of luminescent iridium(III) complexes and investigated their ability to act as luminescent split G-quadruplex probes. After screening, the iridium(III) complex 1 [Ir(2-phenylquinoline)2(3,4,7,8-tetramethyl-1,10-phenanthroline)]PF6 was validated as a highly-selective G-quadruplex probe and was utilized to construct a label-free intermolecular G-quadruplex-based assay for the selective and sensitive detection of LMP1 gene deletion. This "mix-and-detect" assay is simple and selective, and could detect down to 10nM of the target gene in aqueous solution with a linear range from 10 to 500nM. We also investigated the performance of our split G-quadruplex-based sensing platform for LMP1 gene deletion in the presence of cellular debris, demonstrating the robustness of this sensing system in biological samples. Comparative assays were also performed using either organic dyes or labeled oligonucleotides as signal-transducing agents.

Keywords

G-quadruplex, Gene deletion, Iridium(III) complex, Label-free detection, Luminescent probe

Publication Date

2015

Source Publication Title

Biosensors and Bioelectronics

Volume

70

Start Page

338

End Page

344

Publisher

Elsevier

DOI

10.1016/j.bios.2015.03.047

Link to Publisher's Edition

http://dx.doi.org/10.1016/j.bios.2015.03.047

ISSN (print)

09565663

ISSN (electronic)

18734235

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