Document Type

Journal Article

Department/Unit

Department of Biology

Title

Proteomic identification of early salicylate- and flg22-responsive redox-sensitive proteins in Arabidopsis

Language

English

Abstract

Accumulation of reactive oxygen species (ROS) is one of the early defense responses against pathogen infection in plants. The mechanism about the initial and direct regulation of the defense signaling pathway by ROS remains elusive. Perturbation of cellular redox homeostasis by ROS is believed to alter functions of redox-sensitive proteins through their oxidative modifications. Here we report an OxiTRAQ-based proteomic study in identifying proteins whose cysteines underwent oxidative modifications in Arabidopsis cells during the early response to salicylate or flg22, two defense pathway elicitors that are known to disturb cellular redox homeostasis. Among the salicylate- and/or flg22-responsive redox-sensitive proteins are those involved in transcriptional regulation, chromatin remodeling, RNA processing, post-translational modifications, and nucleocytoplasmic shuttling. The identification of the salicylate-/flg22-responsive redox-sensitive proteins provides a foundation from which further study can be conducted toward understanding biological significance of their oxidative modifications during the plant defense response.

Publication Date

2015

Source Publication Title

Scientific Reports

Volume

5

Start Page

8625

Publisher

Nature Publishing Group

DOI

10.1038/srep08625

Link to Publisher's Edition

http://dx.doi.org/10.1038/srep08625

ISSN (print)

20452322

ISSN (electronic)

20452322

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