Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Ratiometric emission fluorescent pH probe for imaging of living cells in extreme acidity

Language

English

Abstract

© 2015 American Chemical Society. A novel ratiometric emission fluorescent probe, 1,1-dimethyl-2-[2-(quinolin-4-yl)vinyl]-1H-benzo[e]indole (QVBI), is facilely synthesized via ethylene bridging of benzoindole and quinoline. The probe exhibits ratiometric fluorescence emission (F522nm/F630nm) characteristics with pKa 3.27 and linear response to extreme-acidity range of 3.8-2.0. Also, its high fluorescence quantum yield (φ = 0.89) and large Stokes shift (110 nm) are favorable. Moreover, QVBI possesses highly selective response to H+ over metal ions and some bioactive molecules, good photostability, and excellent reversibility. The probe has excellent cell membrane permeability and is further applied successfully to monitor pH fluctuations in live cells and imaging extreme acidity in Escherichia coli cells without influence of autofluorescence and native cellular species in biological systems.

Publication Date

2015

Source Publication Title

Analytical Chemistry

Volume

87

Issue

5

Start Page

2788

End Page

2793

Publisher

American Chemical Society

DOI

10.1021/ac504109h

Link to Publisher's Edition

http://dx.doi.org/10.1021/ac504109h

ISSN (print)

00032700

ISSN (electronic)

15206882

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