http://dx.doi.org/10.1002/ptr.5153">
 

Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Title

The puzzling issue of ‘Vehicle-Treated Control’ when using ethanol as drug carrier for MCF-7 cells

Language

English

Abstract

Copyright © 2014 John Wiley & Sons, Ltd.Ethanol has been commonly used as a vehicle for drug discovery purpose in vitro. The human breast cancer MCF-7 estrogen dependent cell line is a common in vitro model used for hormonal therapy study. However, special precaution is suggested when ethanol is used in pharmacological tests as solvent in order to evaluate the biological activity of potential drugs especially concerning about the MCF-7. Ethanol was shown to stimulate the proliferation of this estrogen receptor positive cell line. Here, we have further demonstrated that the dose responsive stimulatory effect of ethanol on the MCF-7 cells after pre-incubating the breast carcinoma cells with phenol red-free medium and stripped fetal bovine serum. Our findings open a discussion for the evaluation of ethanol as solvent for drug discovery and screening when using MCF-7 cells as a testing model.

Keywords

Ethanol, MCF-7 cells, Proliferation, Vehicle

Publication Date

2014

Source Publication Title

Phytotherapy Research

Volume

28

Issue

11

Start Page

1735

End Page

1736

Publisher

Wiley

ISSN (print)

0951418X

ISSN (electronic)

10991573

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