http://dx.doi.org/10.1039/c4tb02104e">
 

Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Directional Plk1 inhibition-driven cell cycle interruption using amphiphilic thin-coated peptide-lanthanide upconversion nanomaterials as in vivo tumor suppressors

Language

English

Abstract

© The Royal Society of Chemistry 2015. Polo-like kinase 1 (Plk1) is a major serine/threonine protein kinase which regulates key mitotic events such as centrosome duplication, spindle assembly and chromosome separation. Overexpression and aberrant activities of Plk1 can be detected in different types of cancer. Given that the unique polo box domain (PBD) pocket provides an excellent drug target for Plk1 binding and inhibition, we have rationally designed multifunctional lanthanide-doped upconversion nanomaterials. NaGdF4:Yb3+, Er3+ (NaGdF4) and BaGdF5:Yb3+, Er3+ (BaGdF5) nanoparticles of two different sizes (60 nm and 10 nm, respectively) have been thin-coated with Plk1 specific peptides (-P1 = PLHSpT, -P2 = PLHSD, and -P3 = GGPLHSpT) to prepare novel nanomaterials. Comparative studies on cellular uptake, anti-cancer activity and imaging properties were then carried out. The experimental data obtained support our original hypothesis that the designed nanomaterials can successfully deliver Plk1 specific peptides into cancer cells causing Plk1 inhibition while simultaneously allowing direct NIR imaging and monitoring. Among the NaGdF4-Pn and BaGdF5-Pn nanoparticle series prepared in this study, NaGdF4-P1 emerged as the best candidate for Plk1 binding and imaging. NaGdF4-P1 can effectively exert cell cycle G2/M arrest and thus selective tumor inhibition both in vitro and in vivo and as such it offers a potentially interesting system for the development of new cancer therapies. This journal is

Publication Date

2015

Source Publication Title

Journal of Materials Chemistry B

Volume

3

Issue

13

Start Page

2624

End Page

2634

Publisher

Royal Society of Chemistry

ISSN (print)

2050750X

ISSN (electronic)

20507518

This document is currently not available here.

Share

COinS