School of Chinese Medicine
Simultaneous determination of ten compounds in rat plasma by UPLC-MS/MS: Application in the pharmacokinetic study of Ma-Zi-Ren-Wan
© 2015 Elsevier B.V. Ma-Zi-Ren-Wan (MZRW) is a classic Chinese formula which has been used to treat human constipation in China for over 2000 years. In order to make good and rational use of this formula in the future, this paper presents the first attempt to track the pharmacokinetic features of MZRW in rat using rapid and sensitive ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Ten chemical components of MZRW, namely, rhein, emodin, aloe emodin, hesperidin, naringin, amygdalin, albiflorin, paeoniflorin, magnolol and honokiol, were simultaneously determined in rat plasma after a single oral administration (10g/kg body weight) of MZRW to rats. Geniposide and liquiritin were used as internal standards. The separation was performed on a Waters ACQUITY BEH C
Active ingredient, Ma-Zi-Ren-Wan, Pharmacokinetics, Rat plasma, UPLC-MS/MS
Source Publication Title
Journal of Chromatography B
Link to Publisher's Edition
Hu, Dong-Dong, Quan-Bin Han, Linda Li-Dan Zhong, Yan-Hong Li, Cheng-Yuan Lin, Hing-Man Ho, Man Zhang, Shu-Hai Lin, Ling Zhao, Tao Huang, Hong Mi, Hong-Sheng Tan, Hong-Xi Xu, and Zhao-Xiang Bian. "Simultaneous determination of ten compounds in rat plasma by UPLC-MS/MS: Application in the pharmacokinetic study of Ma-Zi-Ren-Wan." Journal of Chromatography B 1000 (2015): 136-146.