Document Type

Journal Article

Authors

Dong-Dong Hu, School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Engineering Research Center of Shanghai Colleges for TCM New Drug Discovery
Quan-Bin Han, Lab of Brain and Gut Research, School of Chinese Medicine, Hong Kong Baptist UniversityFollow
Linda Li-Dan Zhong, Lab of Brain and Gut Research, School of Chinese Medicine, Hong Kong Baptist University
Yan-Hong Li, Lab of Brain and Gut Research, School of Chinese Medicine, Hong Kong Baptist University
Cheng-Yuan Lin, Lab of Brain and Gut Research, School of Chinese Medicine, Hong Kong Baptist University
Hing-Man Ho, Lab of Brain and Gut Research, School of Chinese Medicine, Hong Kong Baptist University
Man Zhang, Lab of Brain and Gut Research, School of Chinese Medicine, Hong Kong Baptist University
Shu-Hai Lin, Lab of Brain and Gut Research, School of Chinese Medicine, Hong Kong Baptist UniversityFollow
Ling Zhao, Lab of Brain and Gut Research, School of Chinese Medicine, Hong Kong Baptist University
Tao Huang, Lab of Brain and Gut Research, School of Chinese Medicine, Hong Kong Baptist University
Hong Mi, Lab of Brain and Gut Research, School of Chinese Medicine, Hong Kong Baptist University
Hong-Sheng Tan, School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Engineering Research Center of Shanghai Colleges for TCM New Drug Discovery
Hong-Xi Xu, School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Engineering Research Center of Shanghai Colleges for TCM New Drug Discovery
Zhao-Xiang Bian, Lab of Brain and Gut Research, School of Chinese Medicine, Hong Kong Baptist UniversityFollow

Department/Unit

School of Chinese Medicine

Title

Simultaneous determination of ten compounds in rat plasma by UPLC-MS/MS: Application in the pharmacokinetic study of Ma-Zi-Ren-Wan

Language

English

Abstract

© 2015 Elsevier B.V. Ma-Zi-Ren-Wan (MZRW) is a classic Chinese formula which has been used to treat human constipation in China for over 2000 years. In order to make good and rational use of this formula in the future, this paper presents the first attempt to track the pharmacokinetic features of MZRW in rat using rapid and sensitive ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Ten chemical components of MZRW, namely, rhein, emodin, aloe emodin, hesperidin, naringin, amygdalin, albiflorin, paeoniflorin, magnolol and honokiol, were simultaneously determined in rat plasma after a single oral administration (10g/kg body weight) of MZRW to rats. Geniposide and liquiritin were used as internal standards. The separation was performed on a Waters ACQUITY BEH C18 column (100mm×2.1mm, 1.7μm). The detection was conducted by multiple-reaction monitoring (MRM) in negative ionization mode. Two highest abundant MRM transitions without interference were optimized for each analyte. This method was well validated in terms of linearity, precision, accuracy, recovery, matrix effect and stability. All calibration curves had good linearity (r2>0.995) over the concentration range from 3.9 to 125.0ng/mL for emodin, 3.9-500.0ng/mL for amygdalin, 2.0-4000.0ng/mL for naringin and hesperidin, 3.9-2000.0ng/mL for magnolol, 7.8-2000.0ng/mL for rhein and 3.9-4000.0ng/mL for albiflorin, paeoniflorin, aloe emodin and honokiol. The intra-day and inter-day precision (relative standard deviation) was within 15%, the accuracy (relative error) ranged from -13.6% to 15.1%, and the lower limit of quantification in plasma ranged between 2.0ng/mL and 7.8ng/mL. Extraction recovery, matrix effect and stability were satisfactory. The validated method was successfully applied to a pharmacokinetic study of these ten compounds after oral administration of MZRW to rats. The pharmacokinetic parameters of each compound can facilitate clinical studies in the future.

Keywords

Active ingredient, Ma-Zi-Ren-Wan, Pharmacokinetics, Rat plasma, UPLC-MS/MS

Publication Date

2015

Source Publication Title

Journal of Chromatography B

Volume

1000

Start Page

136

End Page

146

Publisher

Elsevier

DOI

10.1016/j.jchromb.2015.07.003

ISSN (print)

15700232

This document is currently not available here.

Share

COinS