Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

A G-quadruplex-selective luminescent probe with an anchor tail for the switch-on detection of thymine DNA glycosylase activity

Language

English

Abstract

© 2016 Elsevier B.V. Thymine DNA glycosylase (TDG) performs essential functions in maintaining genetic integrity and epigenetic regulation, which also plays an essential role in DNA demethylation. In this work, the novel iridium(III) complex 1 with an anchor tail was synthesized and employed to construct a G-quadruplex-based assay for detecting TDG activity in aqueous solution by using the mismatched base excising property of TDG with T4 DNA ligase and phi29 DNA polymerase, in concert with the rolling circle amplification (RCA) strategy. The assay achieved a detection limit of 0.048 U mL− 1 (0.012 ng mL−1), and showed high selectivity towards TDG even in the presence of other proteins and enzymes. Additionally, the assay could function in diluted cellular debris.

Keywords

G-quadruplex, Iridium(III) complex, Luminescence, TDG

Publication Date

2016

Source Publication Title

Biosensors and Bioelectronics

Volume

86

Start Page

849

End Page

857

Publisher

Elsevier

DOI

10.1016/j.bios.2016.07.082

Link to Publisher's Edition

http://dx.doi.org/10.1016/j.bios.2016.07.082

ISSN (print)

09565663

ISSN (electronic)

18734235

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