Document Type

Journal Article

Authors

Desmond Kwok Po Hau, Research and Development Division, School of Chinese Medicine, Hong Kong Baptist University
Roberto Gambari, BioPharmaNet, Department of Biochemistry and Molecular Biology, The University of Ferrara
Raymond Siu Ming Wong, Department of Medicine and Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong
Marcus Chun Wah Yuen, Institute of Textiles and Clothing and Applied Biology, The Hong Kong Polytechnic University
Gregory Yin Ming Cheng, Department of Medicine and Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong
Cindy Sze Wai Tong, Department of Medicine and Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong
Guo Yuan Zhu, Research and Development Division, School of Chinese Medicine, Hong Kong Baptist University
Alexander Kai Man Leung, Research and Development Division, School of Chinese Medicine, Hong Kong Baptist University
Paul Bo San Lai, Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong
Fung Yi Lau, Department of Medicine and Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong
Andrew Kit Wah Chan, Department of Medicine and Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong
Wai Yeung Wong, Department of Chemistry, Hong Kong Baptist UniversityFollow
Stanton Hon Lung Kok, Institute of Textiles and Clothing and Applied Biology, The Hong Kong Polytechnic University
Chor Hing Cheng, Institute of Textiles and Clothing and Applied Biology, The Hong Kong Polytechnic University
Chi Wai Kan, Institute of Textiles and Clothing and Applied Biology, The Hong Kong Polytechnic University
Albert Sun Chi Chan, Institute of Textiles and Clothing and Applied Biology, The Hong Kong Polytechnic University
Chung Hin Chui, Department of Medicine and Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong
Johnny Cheuk On Tang, Institute of Textiles and Clothing and Applied Biology, The Hong Kong Polytechnic University
David Wang Fun Fong, Research and Development Division, School of Chinese Medicine, Hong Kong Baptist UniversityFollow

Department/Unit

Department of Chemistry

Title

Phyllanthus urinaria extract attenuates acetaminophen induced hepatotoxicity: Involvement of cytochrome P450 CYP2E1

Language

English

Abstract

Acetaminophen is a commonly used drug for the treatment of patients with common cold and influenza. However, an overdose of acetaminophen may be fatal. In this study we investigated whether mice, administered intraperitoneally with a lethal dose of acetaminophen, when followed by oral administration of Phyllanthus urinaria extract, may be prevented from death. Histopathological analysis of mouse liver sections showed that Phyllanthus urinaria extract may protect the hepatocytes from acetaminophen-induced necrosis. Therapeutic dose of Phyllanthus urinaria extract did not show any toxicological phenomenon on mice. Immunohistochemical staining with the cytochrome P450 CYP2E1 antibody revealed that Phyllanthus urinaria extract reduced the cytochrome P450 CYP2E1 protein level in mice pre-treated with a lethal dose of acetaminophen. Phyllanthus urinaria extract also inhibited the cytochrome P450 CYP2E1 enzymatic activity in vitro. Heavy metals, including arsenic, cadmium, mercury and lead, as well as herbicide residues were not found above their detection limits. High performance liquid chromatography identified corilagin and gallic acid as the major components of the Phyllanthus urinaria extract. We conclude that Phyllanthus urinaria extract is effective in attenuating the acetaminophen induced hepatotoxicity, and inhibition of cytochrome P450 CYP2E1 enzyme may be an important factor for its therapeutic mechanism. © 2009 Elsevier GmbH. All rights reserved.

Keywords

Acetaminophen, Cytochrome 450 CYP2E1, Hepatoprotection, Hepatotoxicity, Phyllanthus urinaria

Publication Date

2009

Source Publication Title

Phytomedicine

Volume

16

Issue

8

Start Page

751

End Page

760

Publisher

Elsevier

DOI

10.1016/j.phymed.2009.01.008

ISSN (print)

09447113

ISSN (electronic)

1618095X

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