Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Lighting up cysteine and homocysteine in sequence based on the kinetic difference of the cyclization/addition reaction

Language

English

Abstract

A novel one- and two-photon fluorescent probe CB1 has been developed for discriminating Cys and Hcy in a successive manner with high selectivity. The discrete time-dependent fluorescent responses enable us to sequentially detect Cys and Hcy in different time windows. Two-step reaction and kinetic modes were used to explain the sensing mechanism. As a promising biosensor for cell imaging, CB1 has been confirmed to exhibit membrane permeability to intact cells, low cytotoxicity to viable cells and photostability to ultraviolet light excitation. Furthermore, the results from the control assay have shown that the one- and two-photon fluorescence of CB1 within cells is associated with intracellular mercapto biomolecules but yet there is little interference with physiological pH value, viscosity and common bioanalytes. Finally one- and two-photon fluorescent images of CB1 within living SiHa cells have been presented. © The Royal Society of Chemistry 2013.

Publication Date

2013

Source Publication Title

Organic and Biomolecular Chemistry

Volume

11

Issue

44

Start Page

7721

End Page

7728

Publisher

Royal Society of Chemistry

DOI

10.1039/c3ob41414k

Link to Publisher's Edition

http://dx.doi.org/10.1039/c3ob41414k

ISSN (print)

14770520

ISSN (electronic)

14770539

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