http://dx.doi.org/10.1080/09593330802630801">
 

Document Type

Journal Article

Department/Unit

Department of Biology

Title

Biosurfactants from Acinetobacter calcoaceticus BU03 enhance the solubility and biodegradation of phenanthrene

Language

English

Abstract

A thermophilic bacterial strain, Acinetobacter calcoaceticus BU03, with a biosurfactant-producing capability, was isolated from petroleum-contaminated soil with an improved procedure which employed the solubilization of polycyclic aromatic hydrocarnons (PAHs), i.e. naphthalene in agar plate, as a selection criterion. Crude biosurfactant was recovered from the culture of BU03 by extraction with n-hexane, and its properties were investigated. Biosurfactants from A. calcoaceticus BU03 constitute a thermo-stable mixture, composed of different agents with surface activities. At their critical micelle concentration (CMC) of 152.4 mg L-1, the crude biosurfactants produced from A. calcoaceticus BU03 decreased the air-water surface tension to 38.4 mN m-1. In thermophilic conditions, the emulsifying activity is 2.8 times that of Tween 80. The effects of the biosurfactants produced by A. calcoaceticus on the solubility and biodegradation of PAHs were investigated in batch systems. Biosurfactants produced by A. calcoaceticus BU03 at 25 times their CMC significantly increased the apparent aqueous solubility of phenanthrene (PHE), pyrene (PYR) and benzo(a)pyrene (B[a]P) to 54.3, 6.33 and 2.08mg L-1, respectively. In aqueous system, the biosurfactants at concentrations of 0.5 CMC and 1 CMC slightly enhanced the biodegradation of PHE by a consortium of PAH-degrading microrganisms. Results indicate that biosurfactants from A. calcoaceticus BU03 have potential to enhance the removal of PAHs from contaminated sites. © 2009 Taylor & Francis.

Keywords

Benzo(a)pyrene, Degradation, Isolation, Polycyclic aromatic hydrocarbons, Pyrene, Solubility

Publication Date

2009

Source Publication Title

Environmental Technology

Volume

30

Issue

3

Start Page

291

End Page

300

Publisher

Taylor & Francis

ISSN (print)

09593330

ISSN (electronic)

1479487X

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