Document Type

Journal Article

Department/Unit

Department of Biology

Title

Effects of rhamnolipids on cell surface hydrophobicity of PAH degrading bacteria and the biodegradation of phenanthrene

Language

English

Abstract

The effects of rhamnolipids produced by Pseudomonas aeruginosa ATCC9027 on the cell surface hydrophobicity (CSH) and the biodegradation of phenanthrene by two thermophilic bacteria, Bacillus subtilis BUM and P. aeruginosa P-CG3, and mixed inoculation of these two strains were investigated. Rhamnolipids significantly reduced the CSH of the hydrophobic BUM and resulted in a noticeable lag period in the biodegradation. However, they significantly increased the CSH and enhanced the biodegradation for the hydrophilic P-CG3. In the absence of rhamnolipids, a mixed inoculation of BUM and P-CG3 removed 82.2% of phenanthrene within 30. days and the major contributor of the biodegradation was BUM (rapid degrader) while the growth of P-CG3 (slow degrader) was suppressed. Addition of rhamnolipids promoted the surfactant-mediated-uptake of phenanthrene by P-CG3 but inhibited the uptake through direct contact by BUM. This resulted in the domination of P-CG3 during the initial stage of biodegradation and enhanced the biodegradation to 92.7%. © 2010 Elsevier Ltd.

Keywords

Biodegradation, Biosurfactant, PAHs, Phenanthrene, Rhamnolipids

Publication Date

2011

Source Publication Title

Bioresource Technology

Volume

102

Issue

5

Start Page

3999

End Page

4007

Publisher

Elsevier

DOI

10.1016/j.biortech.2010.11.088

ISSN (print)

09608524

ISSN (electronic)

18732976

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