Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

A highly sensitive G-quadruplex-based luminescent switch-on probe for the detection of polymerase 3′–5′ proofreading activity

Language

English

Abstract

We report herein a luminescent switch-on label-free G-quadruplex-based assay for the rapid and sensitive detection of polymerase proofreading activity using a novel iridium(III) complex as a G-quadruplex-selective probe. The interaction of the iridium(III) complex with the G-quadruplex motif facilitates the highly sensitive switch-on detection of polymerase proofreading activity. Using T4 DNA polymerase (T4 pol) as a model enzyme, the assay achieved high sensitivity and selectivity for T4 pol over other tested enzymes. © 2013 Elsevier Inc.

Keywords

G-quadruplex, Iridium(III) complex, Luminescent, Polymerase, Proofreading

Publication Date

2013

Source Publication Title

Methods

Volume

64

Issue

3

Start Page

224

End Page

228

Publisher

Elsevier

DOI

10.1016/j.ymeth.2013.05.017

Link to Publisher's Edition

http://dx.doi.org/10.1016/j.ymeth.2013.05.017

ISSN (print)

10462023

ISSN (electronic)

10959130

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