Department of Chemistry
A new method for identification of in vitro metabolites of 2,3,7,8-TCDD with rat liver microsomes by using liquid chromatography-mass spectrometry
2,3,7,8-Tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) is known as a highly toxic environmental contaminant and poses potential risks to human and animal health. As a persistent organic pollutant, 2,3,7,8-TCDD is hard to metabolize and thus gets accumulated in the human and animal body. Literature reports on the metabolism study of 2,3,7,8-TCDD are rare. The traditional method of GC-MS for 2,3,7,8-TCDD analysis might not be amenable for the direct analysis of its polar metabolites. In this study, in vitro metabolism of 2,3,7,8-TCDD with rat liver microsomes by using LC-MS and MS/MS was investigated and two hydroxylated metabolites of 2,3,7,8-TCDD and four trichloro-dihydroxydibenzo-p-dioxins were identified from the direct LC-MS and MS/MS analyses of the incubated samples. © 2013 The Royal Society of Chemistry.
Source Publication Title
Royal Society of Chemistry
Wang, Chunyan, Zhi Tang, Yanling Zhang, Yan Liang, Fengrui Song, and Zongwei Cai. "A new method for identification of in vitro metabolites of 2,3,7,8-TCDD with rat liver microsomes by using liquid chromatography-mass spectrometry." Analytical Methods 5.11 (2013): 2757-2760.