Department of Chemistry
An oligonucleotide-based switch-on luminescent probe for the detection of kanamycin in aqueous solution
We report herein the first oligonucleotide-based luminescent method for the sensitive and selective detection of kanamycin in aqueous solution. The assay utilizes a square-planar luminescent platinum(II) complex and the kanamycin aptamer. The luminescence signal of the platinum(II) complex with the oligonucleotide is weak in the absence of kanamycin. Upon the addition of kanamycin, the aptamer changes from a random-coiled structure into a specific conformation containing a hairpin region. This facilitates intercalation of the platinum(II) complex into the bound aptamer, thereby resulting in an enhanced luminescence signal of the system in response to kanamycin. The assay is sensitive and selective towards kanamycin over other antibiotics. The assay could be used to detect trace levels of kanamycin in fish extracts with a comparable sensitivity to aqueous buffer solution. © 2012 Elsevier B.V.
Aptamer, Kanamycin, Luminescent, Platinum(II) complex, Switch-on probe
Source Publication Title
Sensors and Actuators B: Chemical
Link to Publisher's Edition
Leung, Ka-Ho, Hong-Zhang He, Daniel Shiu-Hin Chan, Wai-Chung Fu, Chung-Hang Leung, and Dik-Lung Ma. "An oligonucleotide-based switch-on luminescent probe for the detection of kanamycin in aqueous solution." Sensors and Actuators B: Chemical 177 (2013): 487-492.