Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

An oligonucleotide-based switch-on luminescent probe for the detection of kanamycin in aqueous solution

Language

English

Abstract

We report herein the first oligonucleotide-based luminescent method for the sensitive and selective detection of kanamycin in aqueous solution. The assay utilizes a square-planar luminescent platinum(II) complex and the kanamycin aptamer. The luminescence signal of the platinum(II) complex with the oligonucleotide is weak in the absence of kanamycin. Upon the addition of kanamycin, the aptamer changes from a random-coiled structure into a specific conformation containing a hairpin region. This facilitates intercalation of the platinum(II) complex into the bound aptamer, thereby resulting in an enhanced luminescence signal of the system in response to kanamycin. The assay is sensitive and selective towards kanamycin over other antibiotics. The assay could be used to detect trace levels of kanamycin in fish extracts with a comparable sensitivity to aqueous buffer solution. © 2012 Elsevier B.V.

Keywords

Aptamer, Kanamycin, Luminescent, Platinum(II) complex, Switch-on probe

Publication Date

2013

Source Publication Title

Sensors and Actuators B: Chemical

Volume

177

Start Page

487

End Page

492

Publisher

Elsevier

DOI

10.1016/j.snb.2012.11.053

Link to Publisher's Edition

http://dx.doi.org/10.1016/j.snb.2012.11.053

ISSN (print)

09254005

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