Department of Chemistry
Determination of polychlorinated biphenyls in seawater using headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry with the aid of experimental design
A simple and reliable pretreatment approach in gas chromatography-mass spectrometry (GC-MS) was developed for concurrent clean-up of seawater and the concentration of lipophilic polychlorinated biphenyls (PCBs) with 1-8 chlorine atoms based on the combination of KMnO 4 oxidation with headspace sampling and solid-phase microextraction (SPME). Factors affecting the extraction process were studied using a multivariable approach. Under optimum conditions such as PDMS 7 μm of fiber, 78 °C of extraction temperature, 33 min of extraction time, 8 mL of volume, the limits of detection ranged from 0.3 to 7.5 ng L -1 and precisions were between 3.9 and 9.9% at spiked 0.05 μg L -1 PCBs. Humic acids in seawater exhibited remarkably negative effects; recoveries of PCBs were significantly improved, especially for more lipophilic CB171 and CB201 from 35% and 49% to 78% and 89%, respectively, after KMnO 4 pretreating seawater samples at pH 6. The developed method was demonstrated to be simple, rapid, reliable and applicable for determining different PCBs in seawater containing large amounts of humic substances. © 2012 Sociedade Brasileira de Química.
Experimental design, Gas chromatography-mass spectrometry, Headspace solid-phase microextraction, Humic substances, Polychlorinated biphenyls, Seawater
Source Publication Title
Journal of the Brazilian Chemical Society
Sociedade Brasileira de Química
Link to Publisher's Edition
Song, Xingliang, Jinhua Li, Lingxin Chen, Zongwei Cai, Chunyang Liao, Hailong Peng, and Hua Xiong. "Determination of polychlorinated biphenyls in seawater using headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry with the aid of experimental design." Journal of the Brazilian Chemical Society 23.1 (2012): 132-141.