http://dx.doi.org/10.1590/S0103-50532012000100019">
 

Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Determination of polychlorinated biphenyls in seawater using headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry with the aid of experimental design

Language

English

Abstract

A simple and reliable pretreatment approach in gas chromatography-mass spectrometry (GC-MS) was developed for concurrent clean-up of seawater and the concentration of lipophilic polychlorinated biphenyls (PCBs) with 1-8 chlorine atoms based on the combination of KMnO 4 oxidation with headspace sampling and solid-phase microextraction (SPME). Factors affecting the extraction process were studied using a multivariable approach. Under optimum conditions such as PDMS 7 μm of fiber, 78 °C of extraction temperature, 33 min of extraction time, 8 mL of volume, the limits of detection ranged from 0.3 to 7.5 ng L -1 and precisions were between 3.9 and 9.9% at spiked 0.05 μg L -1 PCBs. Humic acids in seawater exhibited remarkably negative effects; recoveries of PCBs were significantly improved, especially for more lipophilic CB171 and CB201 from 35% and 49% to 78% and 89%, respectively, after KMnO 4 pretreating seawater samples at pH 6. The developed method was demonstrated to be simple, rapid, reliable and applicable for determining different PCBs in seawater containing large amounts of humic substances. © 2012 Sociedade Brasileira de Química.

Keywords

Experimental design, Gas chromatography-mass spectrometry, Headspace solid-phase microextraction, Humic substances, Polychlorinated biphenyls, Seawater

Publication Date

2012

Source Publication Title

Journal of the Brazilian Chemical Society

Volume

23

Issue

1

Start Page

132

End Page

141

Publisher

Sociedade Brasileira de Química

ISSN (print)

01035053

ISSN (electronic)

16784790

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