Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Hit identification of IKKβ natural product inhibitor

Language

English

Abstract

Background: The nuclear factor-κB (NF-κB) proteins are a small group of heterodimeric transcription factors that play an important role in regulating the inflammatory, immune, and apoptotic responses. NF-κB activity is suppressed by association with the inhibitor IκB. Aberrant NF-κB signaling activity has been associated with the development of cancer, chronic inflammatory diseases and auto-immune diseases. The IKK protein complex is comprised of IKKα, IKKβ and NEMO subunits, with IKKβ thought to play the dominant role in modulating NF-κB activity. Therefore, the discovery of new IKKβ inhibitors may offer new therapeutic options for the treatment of cancer and inflammatory diseases.Results: A structure-based molecular docking approach has been employed to discover novel IKKβ inhibitors from a natural product library of over 90,000 compounds. Preliminary screening of the 12 highest-scoring compounds using a luciferase reporter assay identified 4 promising candidates for further biological study. Among these, the benzoic acid derivative (1) showed the most promising activity at inhibiting IKKβ phosphorylation and TNF-α-induced NF-κB signaling in vitro.Conclusions: In this study, we have successfully identified a benzoic acid derivative (1) as a novel IKKβ inhibitor via high-throughput molecular docking. Compound 1 was able to inhibit IKKβ phosphorylation activity in vitro, and block IκBα protein degradation and subsequent NF-κB activation in human cells. Further in silico optimization of the compound is currently being conducted in order to generate more potent analogues for biological tests. © 2013 Leung et al.; licensee BioMed Central Ltd.

Publication Date

2013

Source Publication Title

BMC Pharmacology and Toxicology

Volume

14

Issue

3

Start Page

1

End Page

8

Publisher

BioMed Central

DOI

10.1186/2050-6511-14-3

Link to Publisher's Edition

http://dx.doi.org/10.1186/2050-6511-14-3

ISSN (print)

20506511

ISSN (electronic)

20506511

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