http://dx.doi.org/10.1039/c1an15856b">
 

Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Label-free aptamer-based electrochemical impedance biosensor for 17β-estradiol

Language

English

Abstract

A novel aptamer-based label-free electrochemical impedance spectroscopy biosensor for 17β-estradiol has been fabricated. The aptamers were firstly immobilized on the gold electrode through Au-S interaction; the aptamer probe was then bound with the addition of 17β-estradiol to form the estradiol/aptamer complex on the electrode surface. This leads to a significantly larger interfacial electron transfer resistance than that without the addition of 17β-estradiol. The change in the resistance had a linear relationship with 17β-estradiol concentration in the range of 1.0 × 10 -8 to 1.0 × 10 -11 mol L -1, with a detection limit of 2.0 × 10 -12 mol L -1. The biosensor showed high selectivity to 17β-estradiol and good stability. The designed biosensor has been applied to detect 17β-estradiol in human urine with satisfactory results. © The Royal Society of Chemistry 2012.

Publication Date

2012

Source Publication Title

Analyst

Volume

137

Issue

4

Start Page

819

End Page

822

Publisher

Royal Society of Chemistry

ISSN (print)

00032654

ISSN (electronic)

13645528

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