Department of Chemistry
Label-free aptamer-based electrochemical impedance biosensor for 17β-estradiol
A novel aptamer-based label-free electrochemical impedance spectroscopy biosensor for 17β-estradiol has been fabricated. The aptamers were firstly immobilized on the gold electrode through Au-S interaction; the aptamer probe was then bound with the addition of 17β-estradiol to form the estradiol/aptamer complex on the electrode surface. This leads to a significantly larger interfacial electron transfer resistance than that without the addition of 17β-estradiol. The change in the resistance had a linear relationship with 17β-estradiol concentration in the range of 1.0 × 10 -8 to 1.0 × 10 -11 mol L -1, with a detection limit of 2.0 × 10 -12 mol L -1. The biosensor showed high selectivity to 17β-estradiol and good stability. The designed biosensor has been applied to detect 17β-estradiol in human urine with satisfactory results. © The Royal Society of Chemistry 2012.
Source Publication Title
Royal Society of Chemistry
Link to Publisher's Edition
Lin, Zhenyu, Lifen Chen, Guiyun Zhang, Qida Liu, Bin Qiu, Zongwei Cai, and Guonan Chen. "Label-free aptamer-based electrochemical impedance biosensor for 17β-estradiol." Analyst 137.4 (2012): 819-822.