Department of Chemistry
Label-free aptamer-based electrochemical impedance biosensor for 17β-estradiol
A novel aptamer-based label-free electrochemical impedance spectroscopy biosensor for 17β-estradiol has been fabricated. The aptamers were firstly immobilized on the gold electrode through Au-S interaction; the aptamer probe was then bound with the addition of 17β-estradiol to form the estradiol/aptamer complex on the electrode surface. This leads to a significantly larger interfacial electron transfer resistance than that without the addition of 17β-estradiol. The change in the resistance had a linear relationship with 17β-estradiol concentration in the range of 1.0 × 10 -8 to 1.0 × 10 -11 mol L -1, with a detection limit of 2.0 × 10 -12 mol L -1. The biosensor showed high selectivity to 17β-estradiol and good stability. The designed biosensor has been applied to detect 17β-estradiol in human urine with satisfactory results. © The Royal Society of Chemistry 2012.
Source Publication Title
Royal Society of Chemistry
Lin, Zhenyu, Lifen Chen, Guiyun Zhang, Qida Liu, Bin Qiu, Zongwei Cai, and Guonan Chen. "Label-free aptamer-based electrochemical impedance biosensor for 17β-estradiol." Analyst 137.4 (2012): 819-822.