Document Type

Journal Article

Department/Unit

School of Chinese Medicine; Department of Chemistry; Department of Biology

Language

English

Abstract

Being one of the hallmarks of Alzheimer’s disease, β-amyloid (Aβ) aggregates induce complicated neurotoxicity. Evidences show that the underlying mechanism of neurotoxicity involves a glutamate receptor subtype, N-methyl-D-aspartate (NMDA) receptor, an increase in intracellular calcium(II) ion loading as well as an elevation in oxidation stress. In this work, among the 35 chemical components of Chinese herbal medicines (CHMs) being screened for inhibitors of Aβ aggregation, four of them, namely albiflorin, aloeemodin, neohesperidin and physcion, were found for the first time to exhibit a potent inhibitory effect on Aβ(1-40) and Aβ(1-42) aggregation. Their neuroprotective capability on primary hippocampal neuronal cells was also investigated by MTT assay, ROS assay and intracellular calcium(II) ion concentration measurement. It was interesting to find that physcion was rather toxic to neuronal cells while albiflorin, aloeemodin and neohesperidin reduced the toxicity and ROS induced by both monomeric and oligomeric Aβ species. In addition, albiflorin was particularly powerful in maintaining the intracellular Ca(2+) concentration.

Keywords

Amyloid aggregation, calcium influx, herbs, inhibitors, neuroprotection, reactive oxygen species

Publication Date

2015

Source Publication Title

Current Alzheimer Research

Volume

12

Issue

5

Start Page

424

End Page

433

Publisher

Bentham Science Publishers

Peer Reviewed

1

Copyright

The published manuscript is available at EurekaSelect via http://www.eurekaselect.com/openurl/content.php?genre=article&doi=10.2174/1567205012666150504144919

DOI

10.2174/1567205012666150504144919

ISSN (print)

15672050

ISSN (electronic)

18755828

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