http://dx.doi.org/10.1039/C2AY26232K">
 

Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

L-Ascorbic acid biosensing assay from enzyme-immobilized pig bladder membrane as a novel platform

Language

English

Abstract

An l-ascorbic acid biosensing assay was developed using the enzyme-immobilized pig bladder membrane and dissolved oxygen electrode. The ascorbate oxidase immobilized with chitosan was bound to bladder membrane by glutaraldehyde as a crosslinking agent. The phosphate buffer (100 mM, pH = 5.0), glutaraldehyde concentration (15%), 0.035 mg enzyme loading, and 20-30 °C were established as the optimum test conditions. This biosensor exhibited a response time of 80 s, a generous linear range of 0.010 mM to 0.88 mM with a detection limit of 10 μM, repeatability (3.1%, n = 20), recoveries (98.7-102%), and good stability with a shelf-life of more than 3 months. The reproducibility of fabrication of the biosensors was investigated by using three different membranes (R.S.D. = 3.0%). The comparison of the analytical performance between the published and the proposed biosensor methods was made. This biosensor has been applied to determine the l-ascorbic acid content in real samples, and the results are in agreement with those obtained by a commercial colorimetric ascorbic acid assay kit. © 2013 The Royal Society of Chemistry.

Publication Date

2013

Source Publication Title

Analytical Methods

Volume

5

Issue

5

Start Page

1253

End Page

1258

Publisher

Royal Society of Chemistry

ISSN (print)

17599660

ISSN (electronic)

17599679

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