Department of Chemistry
Layer-by-layer assembly of graphene, Au and poly(toluidine blue O) films sensor for evaluation of oxidative stress of tumor cells elicited by hydrogen peroxide
High level of oxidative stress is involved in formation of incipient tumor and carcinomatous cells. Here in this contribution we have explored a facile strategy to assess the oxidative stress elicited by hydrogen peroxide (H2O2) in cells with amperometric current-time technique in vitro. An electrochemical biosensor exhibiting high sensitivity and selectivity to H2O2 is fabricated by integration of graphene with gold nanoparticles and poly(toluidine blue O) films. The efflux of H2O2 from several representative tumor cells and normal cells on exposure to ascorbic acid could be detected by using the graphene-based nanocomposite films. The results indicate that tumor cells release much more H2O2 than do the normal cells. The novel sensor raises the possibility for clinical diagnostic application to evaluate the higher level of intracellular oxidative stress of tumor cells in comparison with normal cells. © 2012 Elsevier B.V.
Electrochemical detection, Graphene nanocomposites, Layer-by-layer assembly, Oxidative stress, Tumor cells
Source Publication Title
Biosensors and Bioelectronics
Link to Publisher's Edition
Chang, Hucheng, Xuemei Wang, Kowk-Keung Shiu, Yanliang Zhu, Jianling Wang, Qiwei Li, Baoan Chen, and Hui Jiang. "Layer-by-layer assembly of graphene, Au and poly(toluidine blue O) films sensor for evaluation of oxidative stress of tumor cells elicited by hydrogen peroxide." Biosensors and Bioelectronics 41 (2013): 789-794.