Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Layer-by-layer assembly of graphene, Au and poly(toluidine blue O) films sensor for evaluation of oxidative stress of tumor cells elicited by hydrogen peroxide

Language

English

Abstract

High level of oxidative stress is involved in formation of incipient tumor and carcinomatous cells. Here in this contribution we have explored a facile strategy to assess the oxidative stress elicited by hydrogen peroxide (H2O2) in cells with amperometric current-time technique in vitro. An electrochemical biosensor exhibiting high sensitivity and selectivity to H2O2 is fabricated by integration of graphene with gold nanoparticles and poly(toluidine blue O) films. The efflux of H2O2 from several representative tumor cells and normal cells on exposure to ascorbic acid could be detected by using the graphene-based nanocomposite films. The results indicate that tumor cells release much more H2O2 than do the normal cells. The novel sensor raises the possibility for clinical diagnostic application to evaluate the higher level of intracellular oxidative stress of tumor cells in comparison with normal cells. © 2012 Elsevier B.V.

Keywords

Electrochemical detection, Graphene nanocomposites, Layer-by-layer assembly, Oxidative stress, Tumor cells

Publication Date

2013

Source Publication Title

Biosensors and Bioelectronics

Volume

41

Start Page

789

End Page

794

Publisher

Elsevier

DOI

10.1016/j.bios.2012.10.001

Link to Publisher's Edition

http://dx.doi.org/10.1016/j.bios.2012.10.001

ISSN (print)

09565663

ISSN (electronic)

18734235

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