http://dx.doi.org/10.1111/j.1742-4658.2012.08612.x">
 

Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Title

Metabolite profiling of plasma and urine from rats with TNBS-induced acute colitis using UPLC-ESI-QTOF-MS-based metabonomics – a pilot study

Language

English

Abstract

The incidence of inflammatory bowel disease, a relapsing intestinal condition whose precise etiology is still unclear, has continually increased over recent years. Metabolic profiling is an effective method with high sample throughput that can detect and identify potential biomarkers, and thus may be useful in investigating the pathogenesis of inflammatory bowel disease. In this study, using a metabonomics approach, a pilot study based on ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC-ESI-QTOFMS) was performed to characterize the metabolic profile of plasma and urine samples of rats with experimental colitis induced by 2,4,6-trinitrobenzene sulfonic acid. Acquired metabolic profile data were processed by multivariate data analysis for differentiation and screening of potential biomarkers. Five metabolites were identified in urine: two tryptophan metabolites [4-(2-aminophenyl)-2,4-dioxobutanoic acid and 4,6-cihydroxyquinoline], two gut microbial metabolites (phenyl-acetylglycine and p-cresol glucuronide), and the bile acid 12α-hydroxy-3-oxocholadienic acid. Seven metabolites were identified in plasma: three members of the bile acid ? alcohol group (cholic acid, 12a-hydroxy-3-oxocholadienic acid and cholestane- 3,7,12,24,25-pentol) and four lysophosphatidylcholines [LysoPC(20:4), LysoPC( 16:0), LysoPC(18:1) and LysoPC(18:0)]. These metabolites are associated with damage of the intestinal barrier function, microbiota homeostasis, immune modulation and the inflammatory response, and play important roles in the pathogenesis of inflammatory bowel disease. Our results positively support application of the metabonomic approach in study of the pathophysiological mechanism of inflammatory bowel disease. © 2012 The Authors Journal compilation © 2012 FEBS.

Keywords

Experimental inflammatory bowel disease, Metabonomics, Ultra-performance liquid chromatography time-of-flight mass spectrometry

Publication Date

2012

Source Publication Title

FEBS Journal

Volume

279

Issue

13

Start Page

2322

End Page

2338

Publisher

Wiley

ISSN (print)

1742464X

ISSN (electronic)

17424658

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