http://dx.doi.org/10.1039/C2TB00149G">
 

Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Novel composites of multifunctional Fe3O4@Au nanofibers for highly efficient glycoprotein imprinting

Language

English

Abstract

A kind of surface glycoprotein imprinting over magnetic Fe 3O4@Au multifunctional nanofibers (NFs) was developed and investigated. Magnetic Fe3O4@Au nanoparticles (NPs) as the core materials were modified consecutively with aniline, 3-aminophenylboronic acid (APBA) and acrylic acid to introduce boronic acids and polymerizable double bonds. With horseradish peroxidase (HRP) as a glycoprotein template, thin protein-imprinted films were fabricated via radical induced graft copolymerization of monomers on the surface of the multifunctional NFs. Experimental results show that the magnetic multifunctional Fe3O 4@Au NFs can not only direct the selective occurrence of imprinting polymerization, but also drive glycoprotein templates into the polymer through reversible covalent complex formation. The results show that the imprinted NFs reached saturated adsorption at 0.3 mg mL-1 within 90 min and exhibited significant specific recognition towards the template protein. Moreover, the molecularly imprinted polymers (MIPs) were used to electrochemically detect HRP with good linearity in the range of low concentrations from 0.01 to 0.30 mg mL-1 through molecular recognition of K3[Fe(CN)6] current response. The detection limit of this method was found to be 0.005 mg mL-1 (S/N = 3). The synthetic strategy paves the way for preparation of functional nanomaterials by molecular imprinting technique and direct detection of proteins in a more convenient, simpler and cheaper way. © The Royal Society of Chemistry 2013.

Publication Date

2013

Source Publication Title

Journal of Materials Chemistry. B

Volume

1

Issue

7

Start Page

1044

End Page

1051

Publisher

Royal Society of Chemistry

ISSN (print)

2050750X

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