School of Chinese Medicine
“Snow lotus” is a famous Chinese Materia Medica derived from species of the genus Saussurea (Compositae). To differentiate three representative easily-confused snow lotus herbs, namely, Saussurea involucrata (Kar. et Kir.) Sch.-Bip, Saussurea laniceps Hand.-Mazz., and Saussurea medusa Maxim., macroscopic features of the three herbs were systemically observed, and microscopic features were compared by using ordinary light microscopy, polarized light microscopy and scanning electron microscopy (SEM). The results indicate that, as for macroscopic identification, capitula situation and arrangement, and as for microscopic identification, pollen grains, nonglandular hairs, glandular hairs, and cells of inner surface of the microdiodange can be used to authenticate the three snow lotus herbs. Comprehensive table comparing the characteristics were presented in this study. SEM has been found to provide a number of unique characteristics of pollen grains. Based on the observation of pollen grains, evolution sequence of the three species was speculated. The present method was proven to be efficient, convenient, simple, and reliable, which was successfully applied to the authentication of three snow lotus herbs. Microsc. Res. Tech.1 77:631–641, 2014. © 2014 Wiley Periodicals, Inc.
snow lotus, Saussurea, ordinary light microscopy, polarized light microscopy, scanning electron microscopy
Source Publication Title
Microscopy Research and Technique
This is the peer reviewed version of the following article: Chen, Q., Yi, T., Tang, Y., Wong, L. L., Huang, X., Zhao, Z. and Chen, H. (2014), Comparative authentication of three “snow lotus” herbs by macroscopic and microscopic features. Microsc. Res. Tech., 77: 631–641. doi:10.1002/jemt.22381, which has been published in final form at http://dx.doi.org/10.1002/jemt.22381. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
This research is funded by Faculty Research Grant of Hong Kong Baptist University, grant number: FRG/08–09/II-52 and General Research Fund of Hong Kong, grant number: HKBU-260111
Link to Publisher's Edition
Chen, Qilei, Tao Yi, Yina Tang, Lai Lai Wong, Xiaoxuan Huang, Zhongzhen Zhao, and Hubiao Chen. "Comparative authentication of three “snow lotus” herbs by macroscopic and microscopic features." Microscopy Research and Technique 77.8 (2014): 631-641.