Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Simultaneous analysis of strychnine and brucine and their major metabolites by liquid chromatography–electrospray Ion trap mass spectrometry

Language

English

Abstract

A liquid chromatography-electrospray ionization-ion trap mass spectrometry (LC-ESI-ITMS) method was developed for the simultaneous analysis of strychnine, brucine and their major metabolites. Strychnine and brucine were individually incubated with rat liver S9 fraction. The incubation samples were pooled together and analyzed with LC-ESI-ITMS in positive ion and full-scan detection mode. The calibration curves of strychnine and brucine in rat liver showed good linearity in ranges of 0.020 to 8.0 μg/mL for strychnine and 0.020 to 8.5 μg/mL for brucine. The limits of detections were both 0.008 μg/mL and the recoveries were 88.3 and 83.2% for strychnine and brucine, respectively. Two metabolites were identified as strychnine N-oxide and brucine N-oxide by comparing the molecular mass, retention time, full-scan mass spectra, tandem MS and MS3 spectra with those of strychnine and brucine. The developed method provided high sensitivity and selectivity for the determination of poisonous alkaloids and their major metabolites and can be applied in the determination of samples in forensic and clinically toxicological cases. © The Author [2012]. Published by Oxford University Press. All rights reserved.

Publication Date

2012

Source Publication Title

Journal of Analytical Toxicology

Volume

36

Issue

3

Start Page

171

End Page

176

Publisher

Oxford University Press

DOI

10.1093/jat/bks004

Link to Publisher's Edition

http://dx.doi.org/10.1093/jat/bks004

ISSN (print)

01464760

ISSN (electronic)

19452403

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