Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Title

Anti-inflammatory activity of the extracts and fractions from Erigeron multiradiatus through bioassay-guided procedures

Language

English

Abstract

Ethnopharmacological relevance

Erigeron multiradiatus (Lindl.) Benth., an herb that grows in the alpine and subalpine meadow of Qinghai-Tibet plateau, has been widely used as a folk remedy by the native people for treatment of various inflammatory ailments.

Aim of the study

In order to isolate and identify the active components of Erigeron multiradiatus for anti-inflammatory activity, a preliminary phytochemical study and a bioassay-guided fractionation and purification process was performed.

Material and Methods

The dry whole plant Erigeron multiradiatus was extracted with 50% ethanol and then separated into CHCl3, n-BuOH, and aqueous fractions. The anti-inflammatory activities of each fraction were investigated using two in vivo inflammation models.

Results

These results exhibited varying degrees of anti-inflammatory activities and the n-BuOH fraction showed the strongest anti-inflammatory activities. The n-BuOH fraction was then subjected to separation and purification using macroporous resins column chromatography and Sephadex LH-20 leading to two flavonoids glucuronides identified as scutellarein-7-O-β-glucuronide and apigenin-7-O-β-glucuronide. Furthermore, LC–MS/MS identification and quantification of isolated compounds were also performed.

Conclusion

Scutellarein-7-O-β-glucuronide and apigenin-7-O-β-glucuronide were considered as major components and principally responsible for the anti-inflammatory activity of Erigeron multiradiatus. Thus the results of our study provide a scientific basis for the utilization of Erigeron multiradiatus in traditional Tibetan medicine.

Keywords

Erigeron multiradiatus, Anti-inflammatory activity, Flavonoids, LC–MS/MS

Publication Date

9-2008

Source Publication Title

Journal of Ethnopharmacology

Volume

119

Issue

2

Start Page

232

End Page

239

Publisher

Elsevier

Peer Reviewed

1

Funder

The authors thank the Bureau of Science and Technology of Sichuan Province for the support of the applied fundamental study (No. 2006Z08—081). Thanks are also due to The Ministry of National Education Doctoral Fund (No. 20020610089) for providing financial support.

DOI

10.1016/j.jep.2008.06.035

Link to Publisher's Edition

http://dx.doi.org/10.1016/j.jep.2008.06.035

ISSN (print)

03788741

ISSN (electronic)

18727573

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