Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Language

English

Abstract

A new procedure for the separation and isolation of Z-ligustilide from the roots of Angelica sinensis (AS) was developed, and the storage conditions for Z-ligustilide were optimized. Using the present procedure, Z-ligustilide was enriched by decomposing theZ-ligustilide dimers yielding Z-ligustilide and dissolving the polar impurities in hot water. Then, the crude Z-ligustilide was further purified by a semipreparative HPLC system. The spiked and nonspiked samples were used for the evaluation of the proposed procedure. Recoveries obtained varied from 86.2 to 90.7% and RSDs from 4.0 to 6.6%. The yield and purity of the isolated Z-ligustilide were found to be 4.57 mg/g and 99.6%, respectively. The results of stability tests have shown that the presence of oxidant contributes to Z-ligustilide degradation, therefore argon was chosen as a shielding gas for storage. The overall procedure is efficient and convenient which is considered suitable for the preparative separation of Z-ligustilide from AS.

Keywords

Angelica sinensis, Semipreparative HPLC, Shielding gas, Z-Ligustilide

Publication Date

8-2007

Source Publication Title

Journal of Separation Science

Volume

30

Issue

12

Start Page

1973

End Page

1978

Publisher

Wiley

Peer Reviewed

1

Copyright

This is the peer reviewed version of the following article: Yi, T., Zhang, H., Xie, J. and Xue, D. (2007), A new procedure for the preparative separation and isolation of Z-ligustilide from the roots of Angelica sinensis. J. Sep. Science, 30: 1973–1978. doi:10.1002/jssc.200700001, which has been published in final form at http://dx.doi.org/10.1002/jssc.200700001 article using the DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.

DOI

10.1002/jssc.200700001

Link to Publisher's Edition

http://dx.doi.org/10.1002/jssc.200700001

ISSN (print)

16159306

ISSN (electronic)

16159314

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