Document Type

Journal Article

Department/Unit

Department of Chemistry; School of Chinese Medicine

Title

Analysis of Rhizoma Polygoni Cuspidati by HPLC and HPLC-ESI/MS

Language

English

Abstract

An HPLC method with photodiode array detection (PAD) and ESI/MS detection was developed for the qualitative and quantitative analysis of the major chemical constituents of the dried rhizome of Polygonum cuspidatum Sieb. et Zucc. (Rhizoma Polygoni Cuspidati; Chinese name Hu-Zhang). Based on the chromatographic separation on an Altima C18 column using 0.5% aqueous acetic acid and acetonitrile as the mobile phase, nine compounds, including stilbenes, stilbene glucosides, anthraquinones and anthraquinone glucosides, were identified by online ESI/MS analysis and seven were quantified by HPLC-PAD. A full validation of the method including sensitivity, linearity, repeatability and recovery was conducted. Linear calibration was achieved over the concentration range 1–200 mg/L with R2 > 0.999, whilst the limits of detection ranged from 0.51 to 1.57 ng. Repeatability was evaluated by intra- and inter-day assays and the RSD value was within 1.79%. Recoveries of the quantified compounds were within the range 96.0–100.1% with RSD values of less than 2.2%. Five samples of Rhizoma Polygoni Cuspidati from different regions were analysed using the developed method. The major constituents piceid, resveratrol, emodin-8-O-β-d-glucoside and emodin were selected to provide an index for the quality assessment of the herbal drug.

Keywords

HPLC-UV, HPLC-ESI-MS, quality assessment, Polygonum cuspidatum: Rhizoma Polygoni Cuspidati

Publication Date

9-2007

Source Publication Title

Phytochemical Analysis

Volume

18

Issue

5

Start Page

387

End Page

392

Publisher

Wiley

Peer Reviewed

1

DOI

10.1002/pca.993

Link to Publisher's Edition

http://dx.doi.org/10.1002/pca.993

ISSN (print)

09580344

ISSN (electronic)

10991565

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