Document Type

Journal Article

Department/Unit

School of Chinese Medicine; Department of Chemistry

Title

Identification and determination of the major constituents in traditional Chinese medicinal plant Polygonum multiflorum thunb by HPLC coupled with PAD and ESI/MS

Language

English

Abstract

An HPLC–PAD–MS method has been developed for the qualitative and quantitative analysis of the major chemical constituents in Polygonum multiflorum Thunb. Chromatographic separation was conducted on an Alltima C18 column using water:acetonitrile:acetic acid as the mobile phase. Altogether nine compounds of various classes, including stilbene glucosides, anthraquinone glucosides and anthraquinone derivates, were identified by online ESI/MS. Their identities were ascertained by comparison with data derived from the literature and/or standard compounds. Five components were quantified by HPLC–PAD and the method was fully validated. All the linear regressions were acquired with R2 > 0.99 and the quantification limits (with a signal-to-noise ratio of 3) ranged from 0.63 and 1.57 ng. Repeatability was evaluated by intra- and inter-day assays and RSD value was within 2.47%. Recovery studies for the quantified compounds were found to be within the range 96.32–102.53% with RSD less than 2.35%. The overall procedure is rapid and reproducible and is considered suitable for qualitative and quantitative analysis of a large number of samples.

Keywords

HPLC, quality control, phytopharmaceutics, Polygonum multiflorum, Polygonaceae

Publication Date

6-2007

Source Publication Title

Phytochemical Analysis

Volume

18

Issue

3

Start Page

181

End Page

187

Publisher

Wiley

Peer Reviewed

1

DOI

10.1002/pca.963

Link to Publisher's Edition

http://dx.doi.org/10.1002/pca.963

ISSN (print)

09580344

ISSN (electronic)

10991565

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