Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Language

English

Abstract

Pinelliae Rhizoma (PR) is a commonly used Chinese medicinal herb, but it has been frequently reported about its toxicity. According to the traditional Chinese medicine theory, processing can reduce the toxicity of the herbs. Here, we aim to determine if processing reduces the toxicity of raw PR, and to explore the underlying mechanisms of raw PR-induced toxicities and the toxicity-reducing effect of processing. Biochemical and histopathological approaches were used to evaluate the toxicities of raw and processed PR. Rat serum metabolites were analyzed by LC-TOF-MS. Ingenuity pathway analysis of the metabolomics data highlighted the biological pathways and network functions involved in raw PR-induced toxicities and the toxicity-reducing effect of processing, which were verified by molecular approaches. Results showed that raw PR caused cardiotoxicity, and processing reduced the toxicity. Inhibition of mTOR signaling and activation of the TGF-β pathway contributed to raw PR-induced cardiotoxicity, and free radical scavenging might be responsible for the toxicity-reducing effect of processing. Our data shed new light on the mechanisms of raw PR-induced cardiotoxicity and the toxicity-reducing effect of processing. This study provides scientific justifications for the traditional processing theory of PR, and should help in optimizing the processing protocol and clinical combinational application of PR.

Publication Date

10-2016

Source Publication Title

Scientific Reports

Volume

6

Start Page

Article number: 34692 (2016)

Publisher

Nature Publishing Group

Peer Reviewed

1

Copyright

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

Funder

This work was supported by the Research Grants Council of Hong Kong (12125116); the National Natural Science Foundation of China (81673649); Science, Technology and Innovation Commission of Shenzhen (JCYJ20140807091945050, JCYJ20150630164505508 and JCYJ20160229210327924); Guangdong Natural Science Foundation (2016A030313007); Chinese Pharmacopoeia Commission (201207004-10); and the Hong Kong Baptist University (FRG1/15-16/050 and FRG2/15-16/020). We thank our colleagues Prof. Hubiao Chen for providing the picture of Pinellia ternata (Thunb.) Breit., Brian Chi-Yan Cheng and Hiu-Yee Kwan for polishing the language.

DOI

10.1038/srep34692

Link to Publisher's Edition

http://dx.doi.org/10.1038/srep34692

ISSN (print)

20452322

ISSN (electronic)

20452322

JA-5007-29372_suppl.doc (1097 kB)
Supplementary information

Additional Files

JA-5007-29372_suppl.doc (1097 kB)
Supplementary information

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