School of Chinese Medicine
In vitro assays suggest Shenqi Fuzheng Injection has the potential to alter melanoma immune microenvironment
A modern agent Shenqi Fuzheng Injection (SFI), prepared from Codonopsis Radix and Astragali Radix, has been commonly used as a supplementary therapy for cancers including melanoma. This agent was derived from a formula documented in the “National Collection of Chinese Medicine Prescriptions”. The formula has long been used as a remedy for Qi deficiency that is closely associated with cancer-related fatigue and poor quality of life. However, the antimelanoma mechanisms of SFI remain unclear. Here we tested if SFI exerted antimelanoma effects by reprograming the tumour immune microenvironment using in vitroassays.
Materials and methods
The cytotoxic activities of Jurkat T cells when co-cultured with A375 cells were determined in the presence or absence of SFI. The migratory activities of Jurkat T cells were examined in the transwell assay system. The mRNA expression and production of cytokines (IL-10, TGF β and VEGF) in A375 cells in the presence or absence of SFI were determined by real-time PCR and ELISA, respectively.
When A375 cells were co-cultured with Jurkat T cells in the presence of SFI (220 µg/mL), a potent cytotoxicity effect against A375 cells was observed. Supernatants from A375 cells that were treated with SFI (110 and 220 µg/mL) significantly increased the migratory capacity of Jurkat T cells in transwell assays. SFI also markedly reduced the mRNA expression levels and the release of immunosuppressive cytokines IL-10, TGF-β and VEGF in A375 cells in a concentration-dependent manner.
SFI enhanced the cytotoxic and migratory activities of Jurkat T cells towards A375 melanoma cells. The effects were associated with SFI's suppression on immunosuppressive cytokines for their release from and gene expressions in A375 melanoma cells. These in vitro findings suggested that SFI might reprogramme the immunosuppressive melanoma microenvironment in vivo to enhance the cytotoxicity of tumour-infiltrating immune cells. This study provides a pharmacological basis for the adjunctive use of SFI in melanoma treatment.
Source Publication Title
Journal of Ethnopharmacology
© 2016 Elsevier Ireland Ltd. All rights reserved.
This work is partially supported by Livzon Pharmaceutical Group Inc., The Research Grants Council of Hong Kong (12125116) , The Science Technology and Innovation Commission of Shenzhen (JCYJ20140807091945050, JCYJ20150630164505508 and JCYJ20160229210327924), Guangdong Natural Science Foundation (2016A030313007); and Hong Kong Baptist University (FRG1/15–16/050 and FRG2/15–16/020).
Link to Publisher's Edition
Du, Juan, Brian Chi Yan Cheng, Xiu-Qiong Fu, Tao Su, Ting Li, Hui Guo, Su-Mei Li, Jin-Feng Wu, Hua Yu, Wen-Hua Huang, Hui Cao, and Zhi-Ling Yu. "In vitro assays suggest Shenqi Fuzheng Injection has the potential to alter melanoma immune microenvironment." Journal of Ethnopharmacology 194 (2016): 15-19.