http://dx.doi.org/10.1016/j.jhazmat.2010.06.079">
 

Document Type

Journal Article

Authors

Edward H. Sandy, Key Laboratory of Biogeology and Environmental Geology Laboratory of Chinese Ministry of Education, School of Environmental Studies and Sino, Hungarian Joint Laboratory of Environmental Science and Health, China University of Geosciences
Jun Yao, Key Laboratory of Biogeology and Environmental Geology Laboratory of Chinese Ministry of Education, School of Environmental Studies and Sino, Hungarian Joint Laboratory of Environmental Science and Health, China University of Geosciences
Shixue Zheng, State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University
Alhaji B. Gogra, Key Laboratory of Biogeology and Environmental Geology Laboratory of Chinese Ministry of Education, School of Environmental Studies and Sino, Hungarian Joint Laboratory of Environmental Science and Health, China University of Geosciences
Huilun Chen, Key Laboratory of Biogeology and Environmental Geology Laboratory of Chinese Ministry of Education, School of Environmental Studies and Sino, Hungarian Joint Laboratory of Environmental Science and Health, China University of Geosciences
Hui Zheng, State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University
Thomas B.R. Yormah, Chemistry Department, University of Sierra Leone, Fourah Bay College
Xin Zhang, Yale School of Forestry and Environmental Studies, Yale University
Gyula Zaray, Department of Chemical Technology and Environmental Chemistry, Eötvös University
Brunello Ceccanti, Institute of Ecosystem Studies (ISE), Italian National Research Council (ICT-CNR)
Martin M.F. Choi, Department of Chemistry, Hong Kong Baptist UniversityFollow

Department/Unit

Department of Chemistry

Title

A comparative cytotoxicity study of isomeric alkylphthalates to metabolically variant bacteria

Language

English

Abstract

This work investigated the toxicity of two isomeric alkylphthalates, i.e., di-n-octyl phthalate (DOP) and di-2-ethylhexyl phthalate (DEHP) to two model bacteria, Escherichia coli (E. coli) and Bacillus subtilis (B. subtilis), which have been previously used to study the toxicity of environmental pollutants. Microcalorimetry was used as the key analytical tool alongside scanning electron microscopy (SEM) and traditional microbiology techniques. The thermokinetic parameters from microcalorimetry showed that the phthalates had a biphasic effect on the metabolic activities of the bacteria; serving as energy sources for the bacteria thereby stimulating their growth at low dosages (≤150. μg/mL), but displaying inhibitory effects at higher dosages (≥300. μg/mL), indicated by a sharp decrease in growth rate constants at 450. μg/mL. The SEM revealed that the bacterial cells were morphological deformed, with shrunk cells and elongated strands at 600. μg/mL of both phthalates. The elongated strands inferred that the phthalates inhibited the reproductive processes of the bacteria by possibly impeding some stages of cell division. The half inhibitory concentrations of the phthalates showed that DEHP was more toxic than DOP. Additionally, E. coli, a facultative anaerobe, was more susceptible to the toxic effects of phthalates than B. subtilis, an obligate aerobe capable of forming endospores crucial for tolerating extreme environmental conditions. © 2010 Elsevier B.V.

Keywords

Growth inhibition, Microcalorimetry, Phthalates, Toxicity

Publication Date

2010

Source Publication Title

Journal of Hazardous Materials

Volume

182

Issue

3-1

Start Page

631

End Page

639

Publisher

Elsevier

ISSN (print)

03043894

ISSN (electronic)

18733336

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