http://dx.doi.org/10.1016/j.jasms.2009.02.021">
 

Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

A method to enhance a1 ions and application for peptide sequencing and protein identification

Language

English

Abstract

A simple and effective method was developed for peptide sequencing and protein identification through the determination of its N-terminal residue. The method of N-terminal carbamidomethylation with iodoacetamide could specifically and remarkably enhance the intensity of a1 ions in the tandem mass spectra of the peptide derivatives without significantly altering their fragmentation pattern, thus allowing determination of their N-terminal residues. The effectiveness and specificity of the method was demonstrated by confirming and extending sequence interpretation of several model peptides and proteins. The developed method was then applied in the LC-MS/MS analysis of the tryptic digests of myoglobin and a whole protein extract from rat heart tissues. The results from database searches were well validated with the enhancement of a1 ions in tandem mass spectra and the specificity of protein identification was obtained when the information of N-terminal residues was included in the database search. © 2009 American Society for Mass Spectrometry.

Publication Date

2009

Source Publication Title

Journal of the American Society for Mass Spectrometry

Volume

20

Issue

6

Start Page

1214

End Page

1223

Publisher

Springer Verlag

ISSN (print)

10440305

ISSN (electronic)

18791123

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