Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Analysis of aristolochic acids by CE-MS with carboxymethyl chitosan-coated capillary

Language

English

Abstract

A CE-MS method for rapid determination of aristolochic acid-I and aristolochic acid-II (AA-II) in traditional Chinese medicines and biological samples was described in the present paper. AA-I and AA-II can be baseline separated within 6 min by CE-MS with carboxymethyl-chitosan-coated capillary. CZE conditions including pH, concentration of buffer, applied voltage, and capillary temperature were systematically investigated, and the composition and flow rate of sheath liquid were also optimized for CE-MS. Furthermore, the CE-UV method without any additives in BGE solution was established and compared with the CE-MS method. The results showed that the two methods could achieve satisfactory separation efficiency, repeatability, and linearity, while the LOD was 0.6 μmg/mL for CE-UV and 0.05 μmg/mL for CE-MS. Compared with the CE-UV method, the sensitivity of CE-MS was significantly improved, in addition to the structure information provided by MS detection at the same time. As an application example, a spiked sample in human serum was analyzed by the CE-MS method, indicating that the new CE-MS method can be applied to analyze AAs in biological samples. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Keywords

Aristolochic acid, Capillary coating, Carboxymethyl chitosan, CE, MS

Publication Date

2009

Source Publication Title

ELECTROPHORESIS

Volume

30

Issue

10

Start Page

1783

End Page

1789

Publisher

Wiley-VCH Verlag

DOI

10.1002/elps.200800487

Link to Publisher's Edition

http://dx.doi.org/10.1002/elps.200800487

ISSN (print)

01730835

ISSN (electronic)

15222683

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