Department of Chemistry
Chromatographic fingerprint study on Evodia rutaecarpa (Juss.) Benth by HPLC/DAD/ESI-MSn technique
The LC-ESI-MS n method was developed for the analysis and characterization of alkaloids in the extract of E. rutaecarpa (called Wuzhuyu in Chinese). Thirty-six batches of Wuzhuyu from different locations of China were investigated and the common fingerprinting profile was established with a professional analytical software recommended by the State Food and Drug Administration. Fifteen chemical components of the common peaks were identified by multi-stage MS. The effects on the chromatographic profile resulting from different collecting locations, harvesting times or storage times were studied. Hierarchical clustering analysis and principal components analysis were also performed to classify and differentiate the 36 batches of the samples. As a result, those which had same chemical properties were sorted into one cluster, which was very useful in evaluating and controlling the quality of Wuzhuyu. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.
Chromatographic fingerprint, Evodia rutaecarpa (Juss.) Benth, Multi-stage MS
Source Publication Title
Journal of Separation Science
Zhou, Xin, Yang Zhao, Peihai Lei, Zongwei Cai, and Hai Liu. "Chromatographic fingerprint study on Evodia rutaecarpa (Juss.) Benth by HPLC/DAD/ESI-MSn technique." Journal of Separation Science 33.15 (2010): 2258-2265.