Department of Chemistry
Development of a LC–MS/MS method for the determination of antrodin B and antrodin C from Antrodia camphorata extract in rat plasma for pharmacokinetic study
A selective and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the determination of antrodin B and antrodin C in rat plasma. Both target compounds, together with the internal standard (diazepam), were extracted from rat plasma samples by liquid-liquid extraction with ethyl acetate. Chromatographic separation was carried out on an Agilent XDB-C8 column with an isocratic mobile phase consisting of acetonitrile and water (70:30, V/V) at a flow rate of 0.5mL/min. The mass spectrometric detection was performed by selected reaction monitoring (SRM) mode via atmospheric pressure chemical ionization (APCI) source operating in positive ionization mode. The assay exhibited a linear dynamic range of 47.6-4760ng/mL for antrodin B and 56.6-5660ng/mL for antrodin C. The intra- and inter-day precision was less than 5.3% and the accuracy was less than 2.7% for both analytes. The validated method has been applied to the pharmacokinetic study of antrodin B and antrodin C in rats following oral administration of Antrodia camphorata extract. © 2010 Elsevier B.V.
Antrodia camphorata, Antrodin B, Antrodin C, LC-MS/MS, Pharmacokinetics
Source Publication Title
Journal of Pharmaceutical and Biomedical Analysis
Link to Publisher's Edition
Liu, Yongli, Xin Di, Xingchao Liu, Wenjin Shen, and Kelvin Sze-Yin Leung. "Development of a LC–MS/MS method for the determination of antrodin B and antrodin C from Antrodia camphorata extract in rat plasma for pharmacokinetic study." Journal of Pharmaceutical and Biomedical Analysis 53.3 (2010): 781-784.