Department of Chemistry
Fluorescence quenching method for the determination of catechol with gold nanoparticles and tyrosinase hybrid system
The determination method of catechol by fluorescence quenching was developed. The assay was based on the combination of the unique property of gold nanoparticles with tyrosinase enzymatic reaction. In the presence of tyrosinase, the fluorescence of gold nanoparticles was quenched by catechol which can be employed to detect catechol. Under the optimal conditions, a linear range 5.0 × 10-7-1.0 × 10-3 mol L-1 and a detection limit 1.0 × 10-7 mol L-1 of catechol were obtained. o-Quinone intermediate produced from the enzymatic catalyzed oxidation of catechol was considered to play the main role in the fluorescence quenching. © 2009 Chuan Dong.
Catechol, Fluorescence quenching, Gold nanoparticles, Tyrosinase
Source Publication Title
Chinese Chemical Letters
Link to Publisher's Edition
Dong, Wen Juan, Jin Ping Song, Chuan Dong, and Martin M.F. Choi. "Fluorescence quenching method for the determination of catechol with gold nanoparticles and tyrosinase hybrid system." Chinese Chemical Letters 21.3 (2010): 346-348.