Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Fluorescence quenching method for the determination of catechol with gold nanoparticles and tyrosinase hybrid system

Language

English

Abstract

The determination method of catechol by fluorescence quenching was developed. The assay was based on the combination of the unique property of gold nanoparticles with tyrosinase enzymatic reaction. In the presence of tyrosinase, the fluorescence of gold nanoparticles was quenched by catechol which can be employed to detect catechol. Under the optimal conditions, a linear range 5.0 × 10-7-1.0 × 10-3 mol L-1 and a detection limit 1.0 × 10-7 mol L-1 of catechol were obtained. o-Quinone intermediate produced from the enzymatic catalyzed oxidation of catechol was considered to play the main role in the fluorescence quenching. © 2009 Chuan Dong.

Keywords

Catechol, Fluorescence quenching, Gold nanoparticles, Tyrosinase

Publication Date

2010

Source Publication Title

Chinese Chemical Letters

Volume

21

Issue

3

Start Page

346

End Page

348

Publisher

Elsevier

DOI

10.1016/j.cclet.2009.11.043

Link to Publisher's Edition

http://dx.doi.org/10.1016/j.cclet.2009.11.043

ISSN (print)

10018417

ISSN (electronic)

18785964

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