Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Identification of amino acid substitutions in mutated peptides of nucleoprotein from Avian influenza virus

Language

English

Abstract

Nucleoprotein (NP), the structural component of ribonucleoprotein complex of avian influenza virus, performs multiple essential functions in the regulation of viral RNA synthesis and in the control of nuclear traffic of viral proteins. Mutations have often been found in NP, some of which are relevant to viral survival strategies. In this study, we used nanospray-MS/MS to analyze tryptic digestion of nucleoprotein of avian influenza virus (H5N1) and to identify three mutated peptides. The MS/MS analyses allowed the confident determination of the three mutated amino acid residues F313Y, I194V and V408I/L in the mutated peptides of LLQNSQVYSLIRPNENPAHK, GVGTMVMELVR and ASAGQI/LSVQPTFSVQR, respectively. © 2009 Elsevier B.V. All rights reserved.

Keywords

Amino acid substitution, H5N1 virus, Mutated peptide, Nanospray-MS/MS, Nucleoprotein

Publication Date

2009

Source Publication Title

Talanta

Volume

78

Issue

5-4

Start Page

1492

End Page

1496

Publisher

Elsevier

DOI

10.1016/j.talanta.2009.01.057

ISSN (print)

00399140

ISSN (electronic)

18733573

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