Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Isolation of a Methylobacterium organophilium strain, and its application to a methanol biosensor

Language

English

Abstract

A methanol-utilizing strain (ME25) using methanol (MeOH) as the sole carbon source has been isolated from methane-generating pits. ME25 was identified as Methylobacterium organophilium by its physiological characteristics and 16SrDNA sequence. A MeOH biosensor was then developed by immobilizing ME25 along with sensor for dissolved oxygen (O2). Its response is based on the depletion of O2 following oxidation of MeOH by the bacteria. The decrease in O2 is linearly related to the MeOH concentration in the range from 1.6 mg·L-1 to 4800 mg·L-1 and the detection limit for MeOH is 0.27 mg·L-1. The response time of the biosensor is around 20 min. © Springer-Verlag 2009.

Keywords

Dissolved oxygen, Methane-generating pits, Methanol biosensing system, Methanol determination, Methylobacterium organophilium

Publication Date

2009

Source Publication Title

Microchimica Acta

Volume

167

Issue

2-1

Start Page

67

End Page

73

Publisher

Springer Verlag

DOI

10.1007/s00604-009-0214-7

Link to Publisher's Edition

http://dx.doi.org/10.1007/s00604-009-0214-7

ISSN (print)

00263672

ISSN (electronic)

14365073

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