Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Liquid chromatography/mass spectrometry for investigating the biochemical effects induced by aristolochic acid in rats: the plasma metabolome

Language

English

Abstract

In this study, high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry in conjunction with chemometric methods including principal components analysis was used to investigate metabolic profiling in plasma samples from rats dosed with aristolochic acid (AA). Differentiating metabolites were identified by high-resolution mass spectrometry and tandem mass spectrometry analyses, database searching and comparison with the analytical results of authentic standards. Several metabolites in plasma including a glucuronide conjugate, bile acids, lysophosphatidylcholines and fatty acids were detected in rats exposed to AA. To improve visualization, a z-score plot and a cluster heat map were generated for the concentration fluctuations of the metabolites in different dosage groups. The results obtained from this study indicated that class-specific metabolomic patterns were obviously differentiated for each metabolite in the different dosage groups of AA. Copyright (c) 2010 John Wiley & Sons, Ltd.

Publication Date

2010

Source Publication Title

Rapid Communications in Mass Spectrometry

Volume

24

Issue

9

Start Page

1312

End Page

1318

Publisher

Wiley

DOI

10.1002/rcm.4516

Link to Publisher's Edition

http://dx.doi.org/10.1002/rcm.4516

ISSN (print)

09514198

ISSN (electronic)

10970231

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