Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Preparation and evaluation of the highly cross-linked poly(1-hexadecane-co-trimethylolpropane trimethacrylate) monolithic column for capillary electrochromatography

Language

English

Abstract

In this paper, a novel highly cross-linked porous monolithic stationary phase having a long alkyl chain ligand (C16) was introduced and evaluated in CEC. The monolithic stationary phase was prepared by in situ copolymerization of 1-hexadecene, trimethylolpropane trimethacrylate, and 2-acrylamido-2-methyl-1- propanesulfonic acid (AMPS) in the presence of ternary porogenic solvent (cyclohexanol/1,4-butanediol/water). In preparing monoliths, the ternary cross-linker trimethylolpropane trimethacrylate was usually applied to preparing molecularly imprinted polymers or molecularly imprinted solid-phase extraction, instead of binary cross-linker ethylene dimethacrylate. 1-Hexadecene was introduced to provide the non-polar sites (C16) for chromatographic retention, while AMPS was used to generate the EOF for transporting the mobile phase through the monolithic capillary. Monolithic columns were prepared by optimizing proportion of porogenic solvent and AMPS content in the polymerization solution as well as the cross-linkers. The monolithic stationary phases could generate a strong and stable EOF in various pH values and exhibit an RP-chromatographic behavior for neutral compounds. For charged compounds, the separation was mainly based on the association of hydrophobic, electrostatic and electrophoretic interaction. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

Keywords

1-Hexadecene, CEC, Monolithic column, Trimethylolpropane trimethacrylate

Publication Date

2009

Source Publication Title

ELECTROPHORESIS

Volume

30

Issue

20

Start Page

3450

End Page

3457

Publisher

Wiley-VCH Verlag

DOI

10.1002/elps.200900018

Link to Publisher's Edition

http://dx.doi.org/10.1002/elps.200900018

ISSN (print)

01730835

ISSN (electronic)

15222683

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